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superscript ii reverse transcriptase  (TaKaRa)


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    Structured Review

    TaKaRa superscript ii reverse transcriptase
    Superscript Ii Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 734 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/superscript+ii+reverse+transcriptase/PrimeScript+II+Reverse+Transcriptase/pmc12664801-200-12-16
    Average 96 stars, based on 734 article reviews
    superscript ii reverse transcriptase - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Isolation:

    Article Title: Hepatocyte ApoJ accelerates injury-induced liver fibrosis by activation STAT3 through Ranbp2 mediated-SUMOylation.
    Article Snippet: .. The isolated poly(A) RNA 12 was fragmented into smaller segments and then reverse transcribed into complementary 13 DNA (cDNA) with the aid of SuperScript II Reverse Transcriptase (cat# RR036A; 14 Takara). .. Quantitative polymerase chain reaction (qPCR) was carried out using TB 15 Green Premix Ex Taq II (catalog number RR82WR; Takara, Japan) on a CFX96 Touch 16 Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA).

    Article Title: Overexpressing OsNF-YB12 elevated the content of jasmonic acid and impaired drought tolerance in rice.
    Article Snippet: Nuclear factor Y (NF-Y) is an evolutionarily conserved heterotrimeric transcription factor in eukaryotes.. In a previous study, OsNF-YB12 was confirmed to be associated with drought tolerance using the Ecotilling method.. In this study, real-time quantitative RT-PCR revealed that OsNF-YB12 was induced by various abiotic stresses and phytohormones, with expression levels differing between leaves and roots.

    Reverse Transcription:

    Article Title: Hepatocyte ApoJ accelerates injury-induced liver fibrosis by activation STAT3 through Ranbp2 mediated-SUMOylation.
    Article Snippet: .. The isolated poly(A) RNA 12 was fragmented into smaller segments and then reverse transcribed into complementary 13 DNA (cDNA) with the aid of SuperScript II Reverse Transcriptase (cat# RR036A; 14 Takara). .. Quantitative polymerase chain reaction (qPCR) was carried out using TB 15 Green Premix Ex Taq II (catalog number RR82WR; Takara, Japan) on a CFX96 Touch 16 Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA).

    Article Title: AP5Z1 affects hepatocellular carcinoma growth and autophagy by regulating PTEN ubiquitination and modulating the PI3K/Akt/mTOR pathway
    Article Snippet: Using Lipofectamine 3000 (Invitrogen [Thermo Fisher]), we transfected siRNAs into HCC cells. .. We extracted RNA using TRIzol reagent (Invitrogen) and then synthesized complementary deoxyribonucleic acid (cDNA) using SuperScript II reverse transcriptase (TaKaRa Bio, Dalian, China). ..

    Article Title: The versatility of Bacillus tequilensis Bt-CO as an additive: Antagonizing pathogens, enhancing immunity, promoting intestinal health, and optimizing gut microbiota of tilapia
    Article Snippet: The concentration and purity of the extracted RNA were assessed using a Nano-drop ND-1000 spectrophotometer (Nano-Drop Technologies, Wilmington, DE, USA). .. Subsequently, the extracted RNA was reverse transcribed into complementary DNA (cDNA) using Superscript II reverse transcriptase (Takara Biomedical Technology [Beijing] Co., Ltd., Bejing, China). .. RT-qPCR was conducted utilizing a SYBR Green MasterMix (Takara Biomedical Technology [Beijing] Co., Ltd.).

    Article Title: Antibacterial activity of baicalein against Aeromonas hydrophila : in vitro and in vivo evaluation
    Article Snippet: .. Total RNA was extracted using the Trizol method and reverse-transcribed into cDNA with Superscript II reverse transcriptase (Takara, Dalian, China). qRT-PCR was performed using a SYBR Green Pro Taq qPCR Kit (Agbio, Changsha, China) with β -actin as the internal control. ..

    Article Title: The versatility of Bacillus tequilensis Bt-CO as an additive: Antagonizing pathogens, enhancing immunity, promoting intestinal health, and optimizing gut microbiota of tilapia
    Article Snippet: The concentration and purity of the extracted RNA were 335 assessed using a Nano-drop ND-1000 spectrophotometer (Nano-Drop Technologies, 336 Wilmington, DE, USA). .. Subsequently, the extracted RNA was reverse transcribed into 337 complementary DNA (cDNA) using Superscript II reverse transcriptase (Takara 338 Biomedical Technology [Beijing] Co., Ltd., Bejing, China). .. RT-qPCR was conducted 339 utilizing a SYBR Green MasterMix (Takara Biomedical Technology [Beijing] Co., Ltd., 340 Bejing, China).

    Article Title: Genome mining of tailoring enzymes from biosynthetic gene clusters for synthetic biology: A case study with fungal methyltransferases.
    Article Snippet: Poly-A mRNA was isolated with oligo-dT-coupled beads from 40 μg total RNA for each sample (Invitrogen). .. First-strand cDNA was synthesized using random hexamers as primers with Superscript II reverse transcriptase (TaKaRa). .. Exon – intron boundary predictions from FGENESH (http://www. softberry.com/) (Solovyev et al., 2006), antiSMASH Fungal 6.0 (Blin et al., 2021), and NCBI were compared to derive consensus gene models.

    Article Title: AP5Z1 affects hepatocellular carcinoma growth and autophagy by regulating PTEN ubiquitination and modulating the PI3K/Akt/mTOR pathway.
    Article Snippet: Using Lipofectamine 3000 (Invitrogen [Thermo Fisher]), we transfected siRNAs into HCC cells. .. Quantitative reverse-transcription polymerase chain reaction (qRT‒PCR) We extracted RNA using TRIzol reagent (Invitrogen) and then synthesized complementary deoxyribonucleic acid (cDNA) using SuperScript II reverse transcriptase (TaKaRa Bio, Dalian, China). ..

    Article Title: Overexpressing OsNF-YB12 elevated the content of jasmonic acid and impaired drought tolerance in rice.
    Article Snippet: Nuclear factor Y (NF-Y) is an evolutionarily conserved heterotrimeric transcription factor in eukaryotes.. In a previous study, OsNF-YB12 was confirmed to be associated with drought tolerance using the Ecotilling method.. In this study, real-time quantitative RT-PCR revealed that OsNF-YB12 was induced by various abiotic stresses and phytohormones, with expression levels differing between leaves and roots.

    Synthesized:

    Article Title: AP5Z1 affects hepatocellular carcinoma growth and autophagy by regulating PTEN ubiquitination and modulating the PI3K/Akt/mTOR pathway
    Article Snippet: Using Lipofectamine 3000 (Invitrogen [Thermo Fisher]), we transfected siRNAs into HCC cells. .. We extracted RNA using TRIzol reagent (Invitrogen) and then synthesized complementary deoxyribonucleic acid (cDNA) using SuperScript II reverse transcriptase (TaKaRa Bio, Dalian, China). ..

    Article Title: Genome mining of tailoring enzymes from biosynthetic gene clusters for synthetic biology: A case study with fungal methyltransferases.
    Article Snippet: Poly-A mRNA was isolated with oligo-dT-coupled beads from 40 μg total RNA for each sample (Invitrogen). .. First-strand cDNA was synthesized using random hexamers as primers with Superscript II reverse transcriptase (TaKaRa). .. Exon – intron boundary predictions from FGENESH (http://www. softberry.com/) (Solovyev et al., 2006), antiSMASH Fungal 6.0 (Blin et al., 2021), and NCBI were compared to derive consensus gene models.

    Article Title: AP5Z1 affects hepatocellular carcinoma growth and autophagy by regulating PTEN ubiquitination and modulating the PI3K/Akt/mTOR pathway.
    Article Snippet: Using Lipofectamine 3000 (Invitrogen [Thermo Fisher]), we transfected siRNAs into HCC cells. .. Quantitative reverse-transcription polymerase chain reaction (qRT‒PCR) We extracted RNA using TRIzol reagent (Invitrogen) and then synthesized complementary deoxyribonucleic acid (cDNA) using SuperScript II reverse transcriptase (TaKaRa Bio, Dalian, China). ..

    Article Title: Overexpressing OsNF-YB12 elevated the content of jasmonic acid and impaired drought tolerance in rice.
    Article Snippet: Nuclear factor Y (NF-Y) is an evolutionarily conserved heterotrimeric transcription factor in eukaryotes.. In a previous study, OsNF-YB12 was confirmed to be associated with drought tolerance using the Ecotilling method.. In this study, real-time quantitative RT-PCR revealed that OsNF-YB12 was induced by various abiotic stresses and phytohormones, with expression levels differing between leaves and roots.

    Quantitative RT-PCR:

    Article Title: Antibacterial activity of baicalein against Aeromonas hydrophila : in vitro and in vivo evaluation
    Article Snippet: .. Total RNA was extracted using the Trizol method and reverse-transcribed into cDNA with Superscript II reverse transcriptase (Takara, Dalian, China). qRT-PCR was performed using a SYBR Green Pro Taq qPCR Kit (Agbio, Changsha, China) with β -actin as the internal control. ..

    SYBR Green Assay:

    Article Title: Antibacterial activity of baicalein against Aeromonas hydrophila : in vitro and in vivo evaluation
    Article Snippet: .. Total RNA was extracted using the Trizol method and reverse-transcribed into cDNA with Superscript II reverse transcriptase (Takara, Dalian, China). qRT-PCR was performed using a SYBR Green Pro Taq qPCR Kit (Agbio, Changsha, China) with β -actin as the internal control. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Antibacterial activity of baicalein against Aeromonas hydrophila : in vitro and in vivo evaluation
    Article Snippet: .. Total RNA was extracted using the Trizol method and reverse-transcribed into cDNA with Superscript II reverse transcriptase (Takara, Dalian, China). qRT-PCR was performed using a SYBR Green Pro Taq qPCR Kit (Agbio, Changsha, China) with β -actin as the internal control. ..

    Control:

    Article Title: Antibacterial activity of baicalein against Aeromonas hydrophila : in vitro and in vivo evaluation
    Article Snippet: .. Total RNA was extracted using the Trizol method and reverse-transcribed into cDNA with Superscript II reverse transcriptase (Takara, Dalian, China). qRT-PCR was performed using a SYBR Green Pro Taq qPCR Kit (Agbio, Changsha, China) with β -actin as the internal control. ..

    Polymerase Chain Reaction:

    Article Title: AP5Z1 affects hepatocellular carcinoma growth and autophagy by regulating PTEN ubiquitination and modulating the PI3K/Akt/mTOR pathway.
    Article Snippet: Using Lipofectamine 3000 (Invitrogen [Thermo Fisher]), we transfected siRNAs into HCC cells. .. Quantitative reverse-transcription polymerase chain reaction (qRT‒PCR) We extracted RNA using TRIzol reagent (Invitrogen) and then synthesized complementary deoxyribonucleic acid (cDNA) using SuperScript II reverse transcriptase (TaKaRa Bio, Dalian, China). ..



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